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    Pollen Viability of Fraxinus excelsior in Storage Experiments and Investigations on the Potential Effect of Long-Range Transport

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    Buchner, Lisa ; Eisen, Anna-Katharina ; Šikoparija, Branko ; Jochner-Oette, Susanne:
    Pollen Viability of Fraxinus excelsior in Storage Experiments and Investigations on the Potential Effect of Long-Range Transport.
    In: Forests. 13 (2022) 4: 600. - 16 S.
    ISSN 1999-4907

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    Volltext Link zum Volltext (externe URL):
    https://doi.org/10.3390/f13040600

    Kurzfassung/Abstract

    Fragmented ash populations due to ash dieback may lead to a limited gene flow and pollination success. Therefore, the viability of ash pollen plays a major role for the survival of the species. The extent to which the long-distance transport of pollen affects pollen viability was investigated with experiments in a climate chamber using ash pollen samples from a seed orchard in Emmendingen, Germany. Furthermore, experiments with a volumetric pollen trap were conducted. A suitable storage temperature for ash pollen was determined by using four viability tests; TTC test, pollen germination, Alexander’s stain and Acetocarmine. An optimization of the germination medium was performed. We found a strong influence of prevailing temperatures on pollen viability, which decreased faster under warmer conditions. At moderate temperatures, viable pollen could still be observed after 28 days. Thus, a possible successful pollination can also be associated to long-range transported pollen. Storage experiments showed that pollen viability could be maintained longer at temperatures of −20 °C and −80 °C than at 4 °C. In particular, the TTC test has proven to be suitable for determining viability. Therefore, properly stored pollen can be used for breeding programs to support the survival of Fraxinus excelsior.

    Forschungsprojekte

    Quo vadis Pollen? Untersuchungen zur (effektiven) Pollenausbreitung und Pollen- und Samenqualität als Beitrag zur Generhaltung bei der Esche

    Weitere Angaben

    Publikationsform:Artikel
    Schlagwörter:Acetocarmine; Alexander’s stain; Ash dieback; Pollen germination; TTC; Volumetric pollen trap
    Sprache des Eintrags:Englisch
    Institutionen der Universität:Mathematisch-Geographische Fakultät > Geographie > Professur für Physische Geographie/Landschaftsökologie und nachhaltige Ökosystementwicklung
    DOI / URN / ID:10.3390/f13040600
    Open Access: Freie Zugänglichkeit des Volltexts?:Ja
    Peer-Review-Journal:Ja
    Verlag:MDPI
    Die Zeitschrift ist nachgewiesen in:
    Titel an der KU entstanden:Ja
    KU.edoc-ID:30043
    Eingestellt am: 25. Apr 2022 13:57
    Letzte Änderung: 03. Mai 2022 11:29
    URL zu dieser Anzeige: https://edoc.ku.de/id/eprint/30043/
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